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aav9 cag flex tdtomato wpre bgh  (Addgene inc)


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    Structured Review

    Addgene inc aav9 cag flex tdtomato wpre bgh
    Aav9 Cag Flex Tdtomato Wpre Bgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+tdtomato/AAV+pCAG-FLEX-tdTomato-WPRE+(Plasmid+%2351503)/pm41906363-69-0-1
    Average 93 stars, based on 60 article reviews
    aav9 cag flex tdtomato wpre bgh - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia
    Article Snippet: .. DNA plasmid constructs were injected into the lateral ventricle of the fetuses [pCAG-Flag-RhoA-P75S (1.5 μg/μl) plus pCAG-tdTomato (1.5 μg/μl) (Addgene #83029) or control condition with pCAG-tdTomato alone (1.5 to 3.0 μg/μl)]. ..

    Article Title: Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells
    Article Snippet: .. The template for this reaction was pCAG-tdTomato (Addgene plasmid # 83029) and resulted in the construct pCAG-NLS-tdTomato. .. The coding sequence of NLS-tdTOMATO was amplified by PCR with primers 5′-TTCAGATCTTCGGTACCGCCACCATGGGACCTAAGAAAAAGAG-3′ and 5′-TAGATCATCGATGCATCTCGAGTTACTTTTACTTGTACAGCTCGTCC-3′ using pCAG-nls-tdTomato as a template and cloned into the Kpn I- Xho I sites of pPB-CAG-EGFP-puro using the GeneArt Seamless Cloning and Assembly Kit (Thermo Fisher Scientific) to yield pPB-CAG-nls-tdTOMATO-puro.

    Article Title: Dlgap2 deficiency disrupts synaptic homeostasis by promoting ubiquitin-mediated Itsn1 degradation in a valproic acid-induced autism-like model
    Article Snippet: .. Briefly, the pCAG-tdTomato (Addgene, #83029) plasmid DNA was complexed with the Lipofectamine 3000 reagent in Opti-MEM medium and added dropwise to the neuronal cultures. ..

    Article Title: Dlgap2 deficiency disrupts synaptic homeostasis by promoting ubiquitin-mediated Itsn1 degradation in a valproic acid-induced autism-like model.
    Article Snippet: .. Briefly, the pCAG-tdTomato (addgene, #83029) plasmid DNA was complexed with the Lipofectamine 3000 reagent ..

    Article Title: Multimodal Hox5 activity generates motor neuron diversity
    Article Snippet: PCR amplified products and cloning vector (pcDNA3.1-myc-HisA) were digested to create compatible sites for ligation and transformed into NEB10 beta competent bacteria (NEB, Cat# C3019H). .. To create plasmids for chick electroporation, mouse Hoxa5 and Scip cloned into the pcDNA3.1 vector were used to amplify Hoxa5 and Scip and inserted into pCAG-tdTomato (Addgene, Cat #83029), a vector with the chick β-actin promoter/CMV enhancer. .. The complete length of cloned plasmids was sequenced at Eurofins and verified by mapping to the respective mRNAs using the UCSC mouse reference genome.

    Article Title: Working memory features are embedded in hippocampal place fields
    Article Snippet: pAAV-CaMKIIa-hChR2(H134R)-EYFP , Addgene , Plasmid #26969. .. pCAG-tdTomato , Addgene , Plasmid #83029. ..

    Construct:

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia
    Article Snippet: .. DNA plasmid constructs were injected into the lateral ventricle of the fetuses [pCAG-Flag-RhoA-P75S (1.5 μg/μl) plus pCAG-tdTomato (1.5 μg/μl) (Addgene #83029) or control condition with pCAG-tdTomato alone (1.5 to 3.0 μg/μl)]. ..

    Article Title: Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells
    Article Snippet: .. The template for this reaction was pCAG-tdTomato (Addgene plasmid # 83029) and resulted in the construct pCAG-NLS-tdTomato. .. The coding sequence of NLS-tdTOMATO was amplified by PCR with primers 5′-TTCAGATCTTCGGTACCGCCACCATGGGACCTAAGAAAAAGAG-3′ and 5′-TAGATCATCGATGCATCTCGAGTTACTTTTACTTGTACAGCTCGTCC-3′ using pCAG-nls-tdTomato as a template and cloned into the Kpn I- Xho I sites of pPB-CAG-EGFP-puro using the GeneArt Seamless Cloning and Assembly Kit (Thermo Fisher Scientific) to yield pPB-CAG-nls-tdTOMATO-puro.

    Injection:

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia
    Article Snippet: .. DNA plasmid constructs were injected into the lateral ventricle of the fetuses [pCAG-Flag-RhoA-P75S (1.5 μg/μl) plus pCAG-tdTomato (1.5 μg/μl) (Addgene #83029) or control condition with pCAG-tdTomato alone (1.5 to 3.0 μg/μl)]. ..

    Control:

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia
    Article Snippet: .. DNA plasmid constructs were injected into the lateral ventricle of the fetuses [pCAG-Flag-RhoA-P75S (1.5 μg/μl) plus pCAG-tdTomato (1.5 μg/μl) (Addgene #83029) or control condition with pCAG-tdTomato alone (1.5 to 3.0 μg/μl)]. ..

    Amplification:

    Article Title: Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing
    Article Snippet: Fragments were cloned into a pENTR/D-Topo vector (Life Technologies, Carlsbad, CA) using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA) and recombined into a UASp-attB destination vector (gift of F. Wirtz-Peitz) using the Gateway cloning system (Life Technologies, Carlsbad, CA). .. To make pHD-RhoGEF2-tdTomato-Cry2-DsRed, pHD-RhoGEF2-tgRFPt-SspB-DsRed, pHD-RhoGEF2-SspB-DsRed, pHD-SspB-tgRFPt-DsRed-Cysts, and pHD-SspB-DsRed-Cysts, SspB and tgRFPt were amplified from pLL7.0: tgRFPt-SSPB WT (Addgene #60415, gift from Brian Kuhlman), tdTomato was amplified from pCAG-tdTomato (Addgene #83029, gift from Angelique Bordey), Cry2 was amplified from pBI-UASp-mCherry-Cry2phr-RhoGAP71E , DsRed was amplified from pScarlessHD-sfGFP-DsRed (Addgene #80811, gift from Kate O’Connor-Giles), and homology arms were amplified from genomic DNA of vas-Cas9 III (BDSC #51324). ..

    other:

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia.
    Article Snippet: Sections were then washed 3 × 5 min with PBS, incubated with DAPI in 1× PBS for 10 min, washed 3 × 5 min with PBS, and mounted with Prolong Gold antifade mountant (Invitrogen).

    Electroporation:

    Article Title: Multimodal Hox5 activity generates motor neuron diversity
    Article Snippet: PCR amplified products and cloning vector (pcDNA3.1-myc-HisA) were digested to create compatible sites for ligation and transformed into NEB10 beta competent bacteria (NEB, Cat# C3019H). .. To create plasmids for chick electroporation, mouse Hoxa5 and Scip cloned into the pcDNA3.1 vector were used to amplify Hoxa5 and Scip and inserted into pCAG-tdTomato (Addgene, Cat #83029), a vector with the chick β-actin promoter/CMV enhancer. .. The complete length of cloned plasmids was sequenced at Eurofins and verified by mapping to the respective mRNAs using the UCSC mouse reference genome.

    Clone Assay:

    Article Title: Multimodal Hox5 activity generates motor neuron diversity
    Article Snippet: PCR amplified products and cloning vector (pcDNA3.1-myc-HisA) were digested to create compatible sites for ligation and transformed into NEB10 beta competent bacteria (NEB, Cat# C3019H). .. To create plasmids for chick electroporation, mouse Hoxa5 and Scip cloned into the pcDNA3.1 vector were used to amplify Hoxa5 and Scip and inserted into pCAG-tdTomato (Addgene, Cat #83029), a vector with the chick β-actin promoter/CMV enhancer. .. The complete length of cloned plasmids was sequenced at Eurofins and verified by mapping to the respective mRNAs using the UCSC mouse reference genome.



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    Effects of <t>GPR110</t> KO on behavioral outcome. GPR110 WT and KO mice underwent the marble burying test (A) , the open field test (OFT, B–E ), and the novel object recognition test (NORT, F–I ). A total of 50 mice were used for the behavioral experiments, 6 of which were excluded from OFT due to being identified as outliers and 7 excluded from NORT due to spending less than 20 s exploring objects in the familiarization phase. * Describes significant difference at p < 0.05.
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    Effects of <t>GPR110</t> KO on behavioral outcome. GPR110 WT and KO mice underwent the marble burying test (A) , the open field test (OFT, B–E ), and the novel object recognition test (NORT, F–I ). A total of 50 mice were used for the behavioral experiments, 6 of which were excluded from OFT due to being identified as outliers and 7 excluded from NORT due to spending less than 20 s exploring objects in the familiarization phase. * Describes significant difference at p < 0.05.
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    Image Search Results


    Effects of GPR110 KO on behavioral outcome. GPR110 WT and KO mice underwent the marble burying test (A) , the open field test (OFT, B–E ), and the novel object recognition test (NORT, F–I ). A total of 50 mice were used for the behavioral experiments, 6 of which were excluded from OFT due to being identified as outliers and 7 excluded from NORT due to spending less than 20 s exploring objects in the familiarization phase. * Describes significant difference at p < 0.05.

    Journal: Frontiers in Neuroscience

    Article Title: Effects of GPR110 expression on neurobehavioral outcomes in mice

    doi: 10.3389/fnins.2026.1774433

    Figure Lengend Snippet: Effects of GPR110 KO on behavioral outcome. GPR110 WT and KO mice underwent the marble burying test (A) , the open field test (OFT, B–E ), and the novel object recognition test (NORT, F–I ). A total of 50 mice were used for the behavioral experiments, 6 of which were excluded from OFT due to being identified as outliers and 7 excluded from NORT due to spending less than 20 s exploring objects in the familiarization phase. * Describes significant difference at p < 0.05.

    Article Snippet: The pCAG-Lox-STOP-Lox-mouse Adgrf1 -IRES-tdTomato-bGHpolyA was inserted into the C57Bl/6 J mouse ROSA26 locus to create the GPR110 KI mouse (Jackson Laboratories).

    Techniques:

    Physiological and cellular functions predictably inhibited by the changes of protein expression in GPR110 KO mice. Functional (A) and canonical pathway (B) analyses were performed with Ingenuity Pathway Analysis (IPA). Z -scores of ≥2 or ≤ −2 are considered significant.

    Journal: Frontiers in Neuroscience

    Article Title: Effects of GPR110 expression on neurobehavioral outcomes in mice

    doi: 10.3389/fnins.2026.1774433

    Figure Lengend Snippet: Physiological and cellular functions predictably inhibited by the changes of protein expression in GPR110 KO mice. Functional (A) and canonical pathway (B) analyses were performed with Ingenuity Pathway Analysis (IPA). Z -scores of ≥2 or ≤ −2 are considered significant.

    Article Snippet: The pCAG-Lox-STOP-Lox-mouse Adgrf1 -IRES-tdTomato-bGHpolyA was inserted into the C57Bl/6 J mouse ROSA26 locus to create the GPR110 KI mouse (Jackson Laboratories).

    Techniques: Expressing, Functional Assay

    (A) Gene modification for GPR110 knockin (KI) representative sequence. (B) Fold change of GPR110 expression compared to the average expression of WT controls. Outlier test resulted in the exclusion of one WT data point. (C) GPR110 expression in GPR110 KI-VGluT2 hippocampus visualized by TdTomato (red) and with DAPI contrast (blue). * describes significant difference at p < 0.05.

    Journal: Frontiers in Neuroscience

    Article Title: Effects of GPR110 expression on neurobehavioral outcomes in mice

    doi: 10.3389/fnins.2026.1774433

    Figure Lengend Snippet: (A) Gene modification for GPR110 knockin (KI) representative sequence. (B) Fold change of GPR110 expression compared to the average expression of WT controls. Outlier test resulted in the exclusion of one WT data point. (C) GPR110 expression in GPR110 KI-VGluT2 hippocampus visualized by TdTomato (red) and with DAPI contrast (blue). * describes significant difference at p < 0.05.

    Article Snippet: The pCAG-Lox-STOP-Lox-mouse Adgrf1 -IRES-tdTomato-bGHpolyA was inserted into the C57Bl/6 J mouse ROSA26 locus to create the GPR110 KI mouse (Jackson Laboratories).

    Techniques: Modification, Knock-In, Sequencing, Expressing

    Effects of GPR110 overexpression in VGluT2 neurons on behavioral outcome. GPR110 KI-WT and GPR110 KI-VGluT2 mice underwent the marble burying test (A) and the open field test (OFT, B–E ). * describes significant difference at p < 0.05.

    Journal: Frontiers in Neuroscience

    Article Title: Effects of GPR110 expression on neurobehavioral outcomes in mice

    doi: 10.3389/fnins.2026.1774433

    Figure Lengend Snippet: Effects of GPR110 overexpression in VGluT2 neurons on behavioral outcome. GPR110 KI-WT and GPR110 KI-VGluT2 mice underwent the marble burying test (A) and the open field test (OFT, B–E ). * describes significant difference at p < 0.05.

    Article Snippet: The pCAG-Lox-STOP-Lox-mouse Adgrf1 -IRES-tdTomato-bGHpolyA was inserted into the C57Bl/6 J mouse ROSA26 locus to create the GPR110 KI mouse (Jackson Laboratories).

    Techniques: Over Expression

    RNA sequencing analysis of differentially expressed genes upregulated in GPR110 KI-VGluT2 compared to GPR110 KI-WT. GO enrichment analysis of top 30 enriched pathways. The red color represents adjusted p -value of 0.032, while the size of the dot represents the number of DEGs.

    Journal: Frontiers in Neuroscience

    Article Title: Effects of GPR110 expression on neurobehavioral outcomes in mice

    doi: 10.3389/fnins.2026.1774433

    Figure Lengend Snippet: RNA sequencing analysis of differentially expressed genes upregulated in GPR110 KI-VGluT2 compared to GPR110 KI-WT. GO enrichment analysis of top 30 enriched pathways. The red color represents adjusted p -value of 0.032, while the size of the dot represents the number of DEGs.

    Article Snippet: The pCAG-Lox-STOP-Lox-mouse Adgrf1 -IRES-tdTomato-bGHpolyA was inserted into the C57Bl/6 J mouse ROSA26 locus to create the GPR110 KI mouse (Jackson Laboratories).

    Techniques: RNA Sequencing